Protocols
Plasticity Project Protocols Page - TRAP, INTACT, RNA-seq Libaries, etc.
TRAP (Translating Ribosome Affinity Purification) Protocol
Mustroph et al., 2009 Seeds and Constructs from ABRC
DETAILS ORDERING INFO FROM ABRC
It is easier to search for specific lines/constructs on that website.
Plasmids include the TRAP constructs and a number of the pENTR promoter lines.
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Description: Expression construct for immunoprecipitation of ribosomes in pZP111 vector.
Resource type: plasmid
Description: The coding sequence of sGFP was amplified and inserted into p35S:HF-RPL18 (CD3-1628) to generate an expression construct containing the CaMV 35S promo...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
not available
Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...
Resource type: plasmid
Description: pKAT1 promoter driving the expression of HF (His-FLAG tag) reporter and RPL18 (ribosomal protein L18) directed to cotyledon and leaf guard cells. Chlo...
Resource type: plasmid
Description: pLAT52 pollen-specific promoter driving the expression of HF (His-FLAG tag) reporter and RPL18 (ribosomal protein L18. Chloramphenicol resistance in E...
Resource type: plasmid
Description: pGL2 promoter driving the expression of HF (His-FLAG tag), a GFP reporter and RPL18 (ribosomal protein L18) directed to root atrichoblast epidermis, s...
Resource type: plasmid
Description: pCO2 promoter driving the expression of HF (His-FLAG tag), a GFP reporter and RPL18 (ribosomal protein L18) directed to the root cortex meristematic z...
Resource type: plasmid
Description: pSHR shortroot promoter driving the expression of HF (His-FLAG tag) reporter and RPL18 (ribosomal protein L18) directed to root vasculature. Chloramph...
Resource type: plasmid
Description: pRPL11C ribosomal protein 11C promoter driving the expression of HF (His-FLAG tag), a GFP reporter and RPL18 (ribosomal protein L18) directed to root ...
Resource type: plasmid
Description: pSHR shortroot promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.
Resource type: plasmid
Description: pRBCS1A rubisco small subunit promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.
Resource type: plasmid
Description: pCER5 cuticular wax gene promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.
Resource type: plasmid
Description: pSULTR2;2 sulfate transporter promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.
Resource type: plasmid
Description: pGL2 GLABRA2 promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.
Resource type: plasmid
Description: pWOL2 WOODENLEG promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.
Resource type: plasmid
Description: pLAT52 pollen-specific promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.
Resource type: plasmid
Description: pPEP endopeptidase promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.
Resource type: plasmid
Description: pKAT1 potassium channel promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.
Resource type: plasmid
Description: Transgenic line; A construct containing the CaMV 35S promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC...
Resource type: seed
Transgenes: p35S:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the CER5 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...
Resource type: seed
Transgenes: pCER5:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the CO2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...
Resource type: seed
Transgenes: pCO2:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the GL2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...
Resource type: seed
Transgenes: pGL2:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the KAT1 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...
Resource type: seed
Transgenes: pKAT1:HF-RPL1
Genes: AT3G05590
Description: Transgenic line; Ae construct containing the PEP promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...
Resource type: seed
Transgenes: pPEP:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the RBCS promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...
Resource type: seed
Transgenes: pRBCS:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the SCR promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...
Resource type: seed
Transgenes: pSCR:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the SHR promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...
Resource type: seed
Transgenes: pSHR:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the SUC2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...
Resource type: seed
Transgenes: pSUC2:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the Sultr2;2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC...
Resource type: seed
Transgenes: pSultr2;2:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; A construct containing the WOL promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...
Resource type: seed
Transgenes: pWOL:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; The coding sequence of sGFP was amplified and inserted into p35S:HF-RPL18 (CD3-1628) to generate an expression construct containing t...
Resource type: seed
Transgenes: p35S:HF-GFP-RPL18
Genes: AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pCER5:HF-GFP-RPL18
Genes: AT1G51500; AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pCO2:HF-GFP-RPL18
Genes: AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pKAT1:HF-GFP-RPL18
Genes: AT3G05590; AT5G46240
Description: Transgenic line; A construct containing the Solanum lycopersicum LAT52, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR o...
Resource type: seed
Transgenes: pLAT52:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pPEP:HF-GFP-RPL18
Genes: AT1G09750; AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pRBCS:HF-GFP-RPL18
Genes: AT1G67090; AT3G05590
Description: Transgenic line; A construct containing the RPL11C promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC g...
Resource type: seed
Transgenes: pRPL11C:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pRPL11C:HF-GFP-RPL18
Genes: AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pSCR:HF-GFP-RPL18
Genes: AT3G05590; AT3G54220
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...
Resource type: seed
Transgenes: pSUC2:HF-GFP-RPL18
Genes: AT1G22710; AT3G05590
Description: Transgenic line; A construct containing the SULTR2;1 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC...
Resource type: seed
Transgenes: pSULTR2;1:HF-RPL18
Genes: AT3G05590
Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR of the OSC gene fr...
Resource type: seed
Transgenes: pWOL:HF-GFP-RPL18
Genes: AT2G01830; AT3G05590