Protocols

Mustroph et al., 2009 Seeds and Constructs from ABRC

DETAILS ORDERING INFO FROM ABRC

It is easier to search for specific lines/constructs on that website.

Plasmids include the TRAP constructs and a number of the pENTR promoter lines.

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CD3-1628

p35S:HF-RPL18

Description: Expression construct for immunoprecipitation of ribosomes in pZP111 vector.

Resource type: plasmid


CD3-2403

p35S:HF-GFP-RPL18

Description: The coding sequence of sGFP was amplified and inserted into p35S:HF-RPL18 (CD3-1628) to generate an expression construct containing the CaMV 35S promo...

Resource type: plasmid


CD3-2404

pCER5:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2405

pKAT1:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2406

pPEP:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2407

pRBCS:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2408

pSCR:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2409

pSUC2:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2410

pWOL:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2411

pSULTR2;1:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid

not available


CD3-2412

pSHR:HF-GFP-RPL18

Description: To generate this expression construct, a cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR...

Resource type: plasmid


CD3-2735

pKAT1:HF-RPL18

Description: pKAT1 promoter driving the expression of HF (His-FLAG tag) reporter and RPL18 (ribosomal protein L18) directed to cotyledon and leaf guard cells. Chlo...

Resource type: plasmid


CD3-2736

pLAT52:HF-RPL18

Description: pLAT52 pollen-specific promoter driving the expression of HF (His-FLAG tag) reporter and RPL18 (ribosomal protein L18. Chloramphenicol resistance in E...

Resource type: plasmid


CD3-2737

pGL2:HF-GFP-RPL18

Description: pGL2 promoter driving the expression of HF (His-FLAG tag), a GFP reporter and RPL18 (ribosomal protein L18) directed to root atrichoblast epidermis, s...

Resource type: plasmid


CD3-2738

pCO2:HF-GFP-RPL18

Description: pCO2 promoter driving the expression of HF (His-FLAG tag), a GFP reporter and RPL18 (ribosomal protein L18) directed to the root cortex meristematic z...

Resource type: plasmid


CD3-2739

pSHR:HF-RPL18

Description: pSHR shortroot promoter driving the expression of HF (His-FLAG tag) reporter and RPL18 (ribosomal protein L18) directed to root vasculature. Chloramph...

Resource type: plasmid


CD3-2740

pRPL11C:HF-GFP-RPL18

Description: pRPL11C ribosomal protein 11C promoter driving the expression of HF (His-FLAG tag), a GFP reporter and RPL18 (ribosomal protein L18) directed to root ...

Resource type: plasmid


CD3-2741

pSHR in pDONR/Zeo

Description: pSHR shortroot promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.

Resource type: plasmid


CD3-2742

pRBCS1A in pENTR/D-TOPO

Description: pRBCS1A rubisco small subunit promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.

Resource type: plasmid


CD3-2743

pCER5 in pENTR/D-TOPO

Description: pCER5 cuticular wax gene promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.

Resource type: plasmid


CD3-2744

pSULTR2;2 in pDONR/Zeo

Description: pSULTR2;2 sulfate transporter promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.

Resource type: plasmid


CD3-2745

pGL2 in pDONR/Zeo

Description: pGL2 GLABRA2 promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.

Resource type: plasmid


CD3-2746

pWOL in pENTR/D-TOPO

Description: pWOL2 WOODENLEG promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.

Resource type: plasmid


CD3-2747

pLAT52 in pENTR/D-TOPO

Description: pLAT52 pollen-specific promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.

Resource type: plasmid


CD3-2748

pPEP in pENTR/D-TOPO

Description: pPEP endopeptidase promoter in the pENTR/D-TOPO Gateway entry vector. Kanamycin resistance.

Resource type: plasmid


CD3-2749

pKAT1 in pDONR/Zeo

Description: pKAT1 potassium channel promoter in the pDONR/Zeo Gateway entry vector. Zeocin resistance.

Resource type: plasmid


CS66056

Description: Transgenic line; A construct containing the CaMV 35S promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC...

Resource type: seed

Transgenes: p35S:HF-RPL18

Genes: AT3G05590


CS66057

Description: Transgenic line; A construct containing the CER5 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...

Resource type: seed

Transgenes: pCER5:HF-RPL18

Genes: AT3G05590


CS66058

Description: Transgenic line; A construct containing the CO2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...

Resource type: seed

Transgenes: pCO2:HF-RPL18

Genes: AT3G05590


CS66059

Description: Transgenic line; A construct containing the GL2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...

Resource type: seed

Transgenes: pGL2:HF-RPL18

Genes: AT3G05590


CS66060

Description: Transgenic line; A construct containing the KAT1 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...

Resource type: seed

Transgenes: pKAT1:HF-RPL1

Genes: AT3G05590


CS66061

Description: Transgenic line; Ae construct containing the PEP promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...

Resource type: seed

Transgenes: pPEP:HF-RPL18

Genes: AT3G05590


CS66062

Description: Transgenic line; A construct containing the RBCS promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...

Resource type: seed

Transgenes: pRBCS:HF-RPL18

Genes: AT3G05590


CS66063

Description: Transgenic line; A construct containing the SCR promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...

Resource type: seed

Transgenes: pSCR:HF-RPL18

Genes: AT3G05590


CS66064

Description: Transgenic line; A construct containing the SHR promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...

Resource type: seed

Transgenes: pSHR:HF-RPL18

Genes: AT3G05590


CS66065

Description: Transgenic line; A construct containing the SUC2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gen...

Resource type: seed

Transgenes: pSUC2:HF-RPL18

Genes: AT3G05590


CS66066

Description: Transgenic line; A construct containing the Sultr2;2 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC...

Resource type: seed

Transgenes: pSultr2;2:HF-RPL18

Genes: AT3G05590


CS66067

Description: Transgenic line; A construct containing the WOL promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene...

Resource type: seed

Transgenes: pWOL:HF-RPL18

Genes: AT3G05590


CS69096

Description: Transgenic line; The coding sequence of sGFP was amplified and inserted into p35S:HF-RPL18 (CD3-1628) to generate an expression construct containing t...

Resource type: seed

Transgenes: p35S:HF-GFP-RPL18

Genes: AT3G05590


CS69097

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pCER5:HF-GFP-RPL18

Genes: AT1G51500; AT3G05590


CS69098

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pCO2:HF-GFP-RPL18

Genes: AT3G05590


CS69099

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pKAT1:HF-GFP-RPL18

Genes: AT3G05590; AT5G46240


CS69100

Description: Transgenic line; A construct containing the Solanum lycopersicum LAT52, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR o...

Resource type: seed

Transgenes: pLAT52:HF-RPL18

Genes: AT3G05590


CS69101

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pPEP:HF-GFP-RPL18

Genes: AT1G09750; AT3G05590


CS69102

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pRBCS:HF-GFP-RPL18

Genes: AT1G67090; AT3G05590


CS69103

Description: Transgenic line; A construct containing the RPL11C promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC g...

Resource type: seed

Transgenes: pRPL11C:HF-RPL18

Genes: AT3G05590


CS69104

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pRPL11C:HF-GFP-RPL18

Genes: AT3G05590


CS69105

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pSCR:HF-GFP-RPL18

Genes: AT3G05590; AT3G54220


CS69106

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3â UTR of the OSC gene f...

Resource type: seed

Transgenes: pSUC2:HF-GFP-RPL18

Genes: AT1G22710; AT3G05590


CS69107

Description: Transgenic line; A construct containing the SULTR2;1 promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC...

Resource type: seed

Transgenes: pSULTR2;1:HF-RPL18

Genes: AT3G05590


CS69108

Description: Transgenic line; The cassette containing the TMV omega leader sequence, His6-FLAG tag, sGFP tag, coding region of RPL18B and 3 UTR of the OSC gene fr...

Resource type: seed

Transgenes: pWOL:HF-GFP-RPL18

Genes: AT2G01830; AT3G05590